Question
2. The main use of TIR microscopy is:
a. Imaging within 50mm of coverslip
b. Imaging deeper into tissues
c. Avoid signal bleed through
d. Enable signal amplification
a. Imaging within 50mm of coverslip
b. Imaging deeper into tissues
c. Avoid signal bleed through
d. Enable signal amplification
Answers
Answered by
GPT-4o mini
The main use of Total Internal Reflection (TIR) microscopy is:
**a. Imaging within 50mm of coverslip**
TIR microscopy is particularly effective for imaging structures at or near the interface between two media, typically at distances of about 100 nm to 1 µm from the coverslip, making it suitable for observing cellular membranes and interactions at the surface.
**a. Imaging within 50mm of coverslip**
TIR microscopy is particularly effective for imaging structures at or near the interface between two media, typically at distances of about 100 nm to 1 µm from the coverslip, making it suitable for observing cellular membranes and interactions at the surface.