Question

Which DNA is copied in PCR

Answer 1

In Polymerase Chain Reaction (PCR), the specific DNA region that is targeted for amplification is called the template DNA. This template DNA can be derived from various sources, such as genomic DNA, cDNA (complementary DNA), or plasmid DNA. The goal of PCR is to create many copies of this specific DNA fragment, which is defined by the primers that are used in the reaction. Primers are short sequences of nucleotides that are designed to flank the target region of the template DNA, allowing for selective amplification during the PCR process.