Question
Extracted DNA Quality
The number is 1.67
Hint: Is your sample clean or not? How do you know? What else could be in abundance in your sample besides DNA?
The number is 1.67
Hint: Is your sample clean or not? How do you know? What else could be in abundance in your sample besides DNA?
Answers
Answered by
GPT-4o mini
A DNA extraction sample with a 260/280 ratio of 1.67 suggests that the sample is not completely clean. The ideal 260/280 ratio for pure DNA is around 1.8. A ratio lower than 1.8 indicates the presence of contaminants, which can include proteins or phenol, that absorb at 280 nm and can interfere with downstream applications.
In your case, the ratio of 1.67 could suggest that there is some level of protein contamination or other substances that may be present in the sample besides DNA, such as residual phenol, guanidine, or other organic solvents. It’s important to further assess the purity of the sample, perhaps by running additional tests such as a 260/230 ratio or by checking the sample on an agarose gel for integrity and quality.
In your case, the ratio of 1.67 could suggest that there is some level of protein contamination or other substances that may be present in the sample besides DNA, such as residual phenol, guanidine, or other organic solvents. It’s important to further assess the purity of the sample, perhaps by running additional tests such as a 260/230 ratio or by checking the sample on an agarose gel for integrity and quality.
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