Asked by Chinma

ISOLATION OF CHLOROPHYLL AND CAROTENOID PIGMENTS FROM SPINACH:
What would happen to the Rf values of the pigments if you were to increase the relative concentration of acetone in the developing solvent ?

I said the Rf values would be higher because Acetone is more polar than hexane ... so the components would move faster across the TLC plate. We used 70%hexane-30% acetone for preparing our development chamber. ... I would like to know if my answer is right and if not what's the right answer please. Thanks

Answers

Answered by DrBob222
I'm no TLC man but my thinking is the same as yours.
Answered by hmmm
I'm pretty sure pigments travel further when they are less polar. Since acetone is more polar then the rf values would be less.
Answered by Kieu
Thanks
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