To determine the volume of Tramadol Hydrochloride injection needed for an 80 mg dose, you can use the concentration of the stock solution.
The stock solution has a concentration of 50 mg in 2 mL, which can be expressed as:
\[ \text{Concentration} = \frac{50 \text{ mg}}{2 \text{ mL}} = 25 \text{ mg/mL} \]
Now, to find the volume (V) required for an 80 mg dose, use the formula:
\[ \text{Volume (mL)} = \frac{\text{Desired Dose (mg)}}{\text{Concentration (mg/mL)}} \]
Substituting the values:
\[ V = \frac{80 \text{ mg}}{25 \text{ mg/mL}} = 3.2 \text{ mL} \]
Therefore, you will require 3.2 mL of the Tramadol Hydrochloride injection to administer an 80 mg dose.