To analyze the original DNA segment (CCC ATA GCT) and determine which copy exhibits a deletion in the first codon triplet followed by a substitution mutation, we need to first establish what mutations entail:
- Deletion Mutation: This involves the removal of one or more nucleotide bases in the DNA sequence.
- Substitution Mutation: This implies that one base is replaced by another, changing the original sequence.
Now, let's evaluate the responses:
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Response: CCA ATA GCT
- Here, the first codon (CCC) has been changed to (CCA) which indicates a substitution.
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Response: CCA TAA CT
- The first triplet CCC has changed to CCA (substitution), and there appears to be a deletion because there are only five nucleotides instead of nine (the original had three codons).
-
Response: CCC TAG CT
- The first codon appears unchanged (CCC) but we see TAG replacing A in the second codon, followed by deletion of G, making this a substitution with a partial deletion of the last codon.
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Response: CCA TAG CT
- The first codon has still undergone a substitution (CCC to CCA) but doesn’t fully represent the original length.
Given that the question states it specifically requires a deletion in the first codon triplet followed by a substitution, Response 2 (CCA TAA CT) is the correct answer as it shows deletion of the original triplet to a two-nucleotide sequence along with the observed substitutions.