The true statement is: The human body uses 20 amino acids to form all types of proteins.
Here's a brief explanation of the other statements:
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Amino acids form a polypeptide chain via carbon-carbon bonds. - This is incorrect; polypeptide chains are formed by peptide bonds, which are covalent bonds between the carboxyl group of one amino acid and the amino group of another, not carbon-carbon bonds.
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Amino acids do not need to be assembled in a particular order. - This is incorrect; the sequence of amino acids in a polypeptide chain is crucial for the protein's structure and function.
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tRNA is attached to specific proteins in the cell’s cytoplasm. - This is misleading; tRNA (transfer RNA) carries amino acids to the ribosome during protein synthesis and is not generally bound to proteins but interacts with them during translation.