Now, adjust the temperature. What happens when you increase the temperature (1 point)? What happens if you decrease the temperature (1 point)? Do either of the enzymes become denatured with temperature? If so, at what temperatures (1 point)? (3 points)

1 answer

Enzymes are proteins that act as catalysts in biochemical reactions, and their activity is highly sensitive to temperature changes.

  1. Increasing the Temperature: When the temperature is increased, the kinetic energy of the molecules involved in the reaction increases, which generally leads to an increase in the rate of reaction. The enzyme may work more efficiently, as the substrate molecules collide with the active site more frequently.

  2. Decreasing the Temperature: When the temperature is decreased, the kinetic energy of the molecules decreases, which can lead to a slower reaction rate. The enzyme may become less effective at lower temperatures, as there are fewer collisions between the enzyme and substrate.

  3. Denaturation of Enzymes: Yes, enzymes can become denatured with increasing temperature. Denaturation occurs when the three-dimensional structure of the enzyme changes, leading to a loss of function. Most enzymes have an optimal temperature range for activity. Denaturation typically occurs at temperatures significantly above this range, often around 40-60°C for many enzymes, but this can vary depending on the specific enzyme. Some heat-stable enzymes, such as those found in thermophilic organisms, may tolerate higher temperatures without denaturing.

In summary:

  • Increasing Temperature: Increases reaction rate up to a point.
  • Decreasing Temperature: Slows down the reaction rate.
  • Denaturation: Can occur at temperatures usually above 40-60°C for most enzymes.