The variable affecting enzyme function in this experiment is temperature. Specifically, the boiling of beef liver denatures the catalase enzyme, altering its structure and rendering it inactive, which prevents it from breaking down hydrogen peroxide. Enzymes, including catalase, function optimally within a specific temperature range, and extreme temperatures (like boiling) can negatively impact their activity.
A popular lab that can be performed by students is to test the reaction rate of catalase enzyme when it acts on the substrate hydrogen peroxide. Catalase has the ability to break down hydrogen peroxide. Catalase can be found in beef liver from the grocery store! However, if the beef liver is boiled first, the catalase will not be able to break down hydrogen peroxide.
What is the variable affecting enzyme function?
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