Animal sacrifice.

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At the end of the 10-day experimental period, the rats were subjected to an overnight fast. The anesthesia was pertormed on all experimental animals, The anesthetized animals were bled by cardiac puncture and tissues were harvested (liver, kidney, brain etc.). The liver tissue was pounded using mortar and pestle.

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Animal Sacrifice Procedure

At the conclusion of the 10-day experimental period, the rats underwent an overnight fast. Following this, all experimental animals were anesthetized. Once anesthetized, the rats were subjected to cardiac puncture for blood collection, after which various tissues, including the liver, kidneys, and brain, were harvested. The liver tissue was then homogenized using a mortar and pestle for further analysis.