could you check the two pargraph have the dame purpose aims The results indicate that the pMAK-gA + gC combination is particularly effective, achieving precise deletions in 66.6% (4/6) of biallelic deleted clones. HbF reactivation was significantly induced in 18.75% (3/16) and 10% (1/10) of edited clones (#63, #65, and #73). These findings suggest that precise deletion of regulatory regions can significantly elevate HbF levels, potentially offering therapeutic benefits.
In K562, a human erythroleukemia cell line, the specific target distribution of the 200 bp region of the BCL11A gene results in a considerable elevation of γ-globin expression and reproduction of foetal haemoglobin. The statistics demonstrate that pMAK-gA + gC has greater potential for both on-target deletion and HbF reactivation. Of the biallelic deleted clones, 66.6% (4/6) had the exact expected deletion. Following transfection with gA+gC, 18.75% (3/16) and 10% (1/10) of the genetically altered clones (#63, #65, and #73) exhibited significant induction of foetal haemoglobin reactivation.
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Yes, both paragraphs have the same purpose and aim, which is to discuss the effectiveness of the pMAK-gA + gC combination in achieving precise deletions in regulatory regions that can lead to an elevation of HbF levels in cells. They both present similar results and highlight the potential therapeutic benefits of using this combination for HbF reactivation.