1. Size of the molecule: Larger molecules will move more slowly through the gel, resulting in a lower electrophoretic mobility.
2. Charge of the molecule: Molecules with a higher charge will move more quickly through the gel, resulting in a higher electrophoretic mobility.
3. Strength of the electric field: A stronger electric field will exert a greater force on the molecules, increasing their mobility.
4. Medium in which the electrophoresis is performed: The composition of the gel or buffer used in the electrophoresis can affect the mobility of the molecules.
5. pH of the medium: The pH of the medium can affect the charge of the molecule, which in turn affects its electrophoretic mobility.
6. Temperature: Higher temperatures can increase the mobility of molecules through the gel.
7. Presence of additives: The presence of detergents or other additives can affect the mobility of the molecules in electrophoresis.
8. Purity of the sample: Contaminants or impurities in the sample can affect the mobility of the molecules in electrophoresis.
Factors that affects electrophoretic mobility
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