Multiple Choice 1: False
Multiple Choice 2: oxidase negative
Multiple Choice 3: False
Oxidase Test
Read the report and answer all questions.
The oxidase test detects the presence of the enzyme cytochrome oxidase, which is an important component of the electron transport chain functioning in aerobic respiration. This test can be performed in several different ways, with the easiest method consisting of using a cotton swab to pick up an isolated colony from an agar plate and then dropping oxidase reagent (tetramethyl-p-phenylenediamine) onto the swab. A positive test is indicated by a darkening (blue back) of the cotton tip. A negative test is indicated by a cream to yellow color.
A ear buds test for Oxidase positive and negative test.Negative Oxidase test appears as brown color coating in ear buds. Positive Oxidase test appears as blue color dipped in ear buds.
Photos © McGraw-Hill Education, Gabriel Guzman photographer.
The oxidase test requires the use of a reagent called Kovac's reagent.
multiple choice 1
True
False
If a colony is selected with a swab and the addition of oxidase reagent results in no color change, how is this result interpreted?
multiple choice 2
oxidase negative
oxidase positive
The cytochrome oxidase enzyme is a key enzyme catalyzing reactions in the lactic acid fermentation pathway.
multiple choice 3
True
False
1 answer
1 answer