Question

15. Name and discuss a source of error in performing and evaluating gel electrophoresis.

Answer 1

One source of error in performing and evaluating gel electrophoresis is sample contamination. Contaminants such as salts, proteins, or nucleic acids from the sample can interfere with the migration of the DNA fragments through the gel, leading to distorted or inaccurate results. This can happen if the sample is not properly purified before loading it onto the gel, or if the gel equipment or buffer solutions are not properly cleaned and maintained. To minimize this error, it is important to handle samples carefully, use clean equipment, and regularly clean and maintain the gel electrophoresis system.