Question

A laboratory technologist was working on ELISA for measurement of HIV gp24, the standard was checked and was working nicely however serial dilution of the standard showed the absorbance of the standard was decreased when standard concentration is increasing, what is the possible source of error A.standard solution was not done serially B. standard solution was done serially im the correct order c. standard solution was done serially im the reverse order D. Standard dilute to diluent ratio was mistaken but the order was correct

Answer 1

The possible source of error in this case is option D. Standard dilute to diluent ratio was mistaken but the order was correct.