Question

You wish to measure the change in G°'for the hydrolysis of ATP:

ATP ---> ADP + Pi

but the equilibrium for the hydrolysis lies so far toward products that analysis of the ATP concentration at equilibrium is neither practical nor accurate. However, you have the following data that will allow calculation of the value indirectly.

K'eq = 59.5
change in G°'= + 43.0 kJ/mole

Reaction 1: Creatine phosphate + ADP-->
ATP + creatine

Reaction 2: Creatine + Pi-->creatine phosphate

1. Calculate the change in G°'for rxn 1

2. Calculate the change in G°'for the hydrolysis of ATP

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