is third base of codon doesn't specified for a single amino acids and which base is responsible for specification

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The third base of a codon is not always specified for a single amino acid. This is because of a genetic phenomenon called "wobble" or degeneracy, which allows multiple codons to code for the same amino acid.

The third base of a codon is often referred to as the "wobble position" because it has less impact on the amino acid specified. In this position, there is flexibility, and certain non-standard base pairings can occur. This means that a single amino acid can be specified by multiple codons that differ only in their third base.

For example, the amino acid leucine can be specified by six different codons: UUA, UUG, CUU, CUC, CUA, and CUG. The first two bases of these codons are the same, while the third base varies, but they all code for leucine.

Therefore, the third base of a codon is responsible for providing flexibility in the genetic code, allowing different codons to specify the same amino acid.
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