what is dideoxysequencing?
1 answer
Dideoxysequencing, also known as Sanger sequencing, is a method used to determine the nucleotide sequence of DNA. This technique involves adding dideoxynucleotides (ddNTPs) to the DNA synthesis reaction, which terminate the extension of the DNA chain at specific positions. The resulting fragments are separated by size using gel electrophoresis and their sequence can be determined by reading the band pattern from the gel. This method was developed by Frederick Sanger in the 1970s and has been widely used for DNA sequencing ever since. It played a key role in the Human Genome Project, which aimed to determine the complete DNA sequence of the human genome.
1 answer