Terminator sequencing, also known as fluorescent dye terminator sequencing, is a method used to sequence DNA. The DNA to be sequenced is first amplified using the polymerase chain reaction (PCR) to amplify it. The DNA is then mixed with short fragments of DNA known as primers, which bind to the beginning of the DNA sequence being analyzed.
Next, DNA polymerase is used to add fluorescent dyes to the DNA as the sequencing proceeds. Each of the four DNA nucleotides (adenine, thymine, cytosine, and guanine) is labeled with a different color of fluorescent dye. The sequencing reaction is then stopped using a chemical termination process.
The DNA fragments are separated by size using gel electrophoresis, with smaller fragments migrating further down the gel than longer ones. The fluorescent dyes are excited using a laser, and the emitted light is captured by a detector. The sequence of the DNA can then be read by analyzing the colors of the fluorescent dyes that are detected at each step.
Terminator sequencing is a highly accurate and efficient method for sequencing DNA, and is commonly used in research, clinical, and forensic applications.
terminator sequencing of a dna
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