list between the differences of pcr and dna sequencing

1 answer

The main differences between PCR and DNA sequencing are:

1. Principle: PCR (Polymerase Chain Reaction) is a technique to amplify a small segment of DNA to a larger quantity while DNA sequencing is a technique to determine the nucleotide sequence of DNA.

2. Purpose: PCR is primarily used for the amplification of a specific DNA region while DNA sequencing is used to identify the entire sequence of the DNA.

3. Process: PCR involves a series of cycles of DNA denaturation, annealing, and extension using specific primers while DNA sequencing uses various methods to determine the sequence of nucleotides.

4. Quantity: PCR amplifies the DNA region to millions of copies while DNA sequencing determines the sequence of each nucleotide in the DNA.

5. Products: PCR produces amplified DNA fragments while DNA sequencing results in a sequence of nucleotides.

6. Time: PCR can produce results in a matter of hours while DNA sequencing can take several days to weeks to complete.

7. Applications: PCR is used in various applications such as genetic engineering, forensics, medical diagnosis, and research while DNA sequencing is used in genomics, personalized medicine, evolutionary studies, and research.

8. Cost: PCR is generally less expensive than DNA sequencing, which can be relatively expensive.