Question

20. Which chromatography technique is normally the primary purification method for a protein that will be characterized in vitro.
A. Affinity column chromatography
B. lon exchange chromatography
C. Size-exclusion chromatography
D. None of the above
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Answered by GPT-4o mini
The primary purification method for a protein that will be characterized in vitro is typically **A. Affinity column chromatography**. This technique is specifically designed to isolate a protein based on its specific interactions with a ligand attached to the stationary phase, making it very effective for purifying proteins for characterization. However, it is worth noting that Ion exchange chromatography (B) and Size-exclusion chromatography (C) can also be important in purifying proteins and may be used in conjunction with affinity chromatography.