16. The following are all steps in the production of a bacterium having recombinant DNA, which includes an inserted nonbacterial gene. They are in random order.

Question

1. gel electrophoresis of plasmid DNA from bacteria in colonies showing antibiotic resistance
2. sticky ends are allowed to pair up
3. a restriction endonuclease is used to remove the gene to be inserted from its source and also to cut open a plasmid that includes a gene for antibiotic resistance
4. the bacterial colonies are treated with antibiotic
5. treatment with ligase
6. transformation
The correct order of these steps is:
a. 2,5,1,3,4,6
b. 4,3,6,2,5,1
c. 5,1,2,3,4,6
d. 3,2,5,6,4,1
e. 6,3,5,2,4,1

Answer 1

b. 4,3,6,2,5,1