Asked by Lyndsay
A dye has a λmax = 460 nm (ε = 70,000 L/(mol*cm)). What is the concentration of dye in a sample whose absorbance at 460 nm is A = 0.350 in a cell with a path length of 1.00 cm (4 points)
I have no idea how to do this.
I have no idea how to do this.
Answers
Answered by
DrBob222
A = ebc where A is absorbance, e is molar absorptivity constant, b is path length in cm and c is concentration in moles/L.
Substitute for A, e, b, and solve for c in M. You have all of the numbers in the problem.
Substitute for A, e, b, and solve for c in M. You have all of the numbers in the problem.
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