Question

1.You have a mixture of protein A and protein B in a buffer of pH 7. The pI of protein A is 3 and the pI of protein B is 8. The molecular weight is the same. (a) What kind of column chromatograph would you use to separate them? Explain your answer in 2-3 sentences. (b) Can you use SDS PAGE to confirm the purity?(c) Explain your answer

Answer 1

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