The line "And each separate dying ember wrought its ghost upon the floor" suggests a haunting, melancholic atmosphere that emphasizes themes of loss and memory. The most suitable response from the given options is:
D The dying of the fire is making the speaker think of the death of Lenore, and the possible appearance of her ghost.
This option captures the connection between the fading embers of the fire and the speaker's thoughts of Lenore, evoking feelings of grief and the spectral presence of his lost love.