Question

 

17. You need to conduct an experiment in the laboratory. This requires that you prepare 500 ml of  sodium acetate buffer with pH = 4.30. In laboratory you have  solution of CH3COOH (pKa = 4.75), and a stock of CH3COONa.3H2O (MW=136.082 g/mol). Using the Henderson-Hasselbach equation and the mass balance and the ionization equation below, calculate how to prepare this buffer. Assume that the preparation consists of weighing out the necessary amounts of the compounds, or pipetting the required volume of the stock solution available. These are subsequently dissolved in water, and the buffer is prepared in a volumetric flask.