Asked by chemstudent
Is this the wrong forum for these type of questions or can I improve the way I'm asking. If so, let me know. Thanks :)
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A quantitative amino acid analysis reveals that bovine serum albumin (BSA) contains 0.58% tryptophan (Mr 204) by weight.
A) Calculate the minimum molecular weight of BSA (i.e. assume there is only one Trp residue per protein molecule)
B) Gel filtration of BSA gives a molecular weight estimate of 70,000. How many Trp residues are present in a molecule of serum albumin?
In the back of the book, the answers are:
A) 32000
B) 2
I got
A) 204/.58 = 352 daltons.
B) 70000*.58/204 = 199 Trp residues
My calculations seems correct, and I can't see what I did wrong. Can anyone help? Thanks!
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A quantitative amino acid analysis reveals that bovine serum albumin (BSA) contains 0.58% tryptophan (Mr 204) by weight.
A) Calculate the minimum molecular weight of BSA (i.e. assume there is only one Trp residue per protein molecule)
B) Gel filtration of BSA gives a molecular weight estimate of 70,000. How many Trp residues are present in a molecule of serum albumin?
In the back of the book, the answers are:
A) 32000
B) 2
I got
A) 204/.58 = 352 daltons.
B) 70000*.58/204 = 199 Trp residues
My calculations seems correct, and I can't see what I did wrong. Can anyone help? Thanks!
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