Asked by Jenna
how to prepare 500ml of a 0.75M Sodium Phosphate buffer solution at pH 7.5, how would you go about doing that?
Answers
Answered by
DrBob222
I like to do these in millimoles. Although technically they are to be done with concentration in molarity, mmols works just as well and I think it is easier.
You have two equations. Let a = acid and b = base.
eqn 1 is a + b = 500 x 0.75 mmols.
eqn 2 is 7.5 = pKa2 + log (b/a)
where pKa2 is that of H3PO4.
Solve eqn 1 and eqn 2 simultaneously for a and b in millimoles.
Then convert mmols acid and mmols base to grams, weigh each and make the solution. The acid will be NaH2PO4 and the base will be Na2HPO4.
You have two equations. Let a = acid and b = base.
eqn 1 is a + b = 500 x 0.75 mmols.
eqn 2 is 7.5 = pKa2 + log (b/a)
where pKa2 is that of H3PO4.
Solve eqn 1 and eqn 2 simultaneously for a and b in millimoles.
Then convert mmols acid and mmols base to grams, weigh each and make the solution. The acid will be NaH2PO4 and the base will be Na2HPO4.
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