Question

DrBob222, it is me again. Continue the question
5mL of a solution A (unknown concentration) was transferred into sic 25mL volumetric flask. The following volumes of a standard solution of A with with a concentration 75ppm were added to the flask: 0mL, 0.5mL, 1mL, 1.5mL, 2mL, and 2.5L. The excitation spectrum and and emission spectra is provided. Determine the unknown concentration of A in that solution?

I was given excitation spectrum of A and fluorescence spectra of solution #1 to #6
Thanks to your suggestion, my final answer is 7.48447 ppm (unknown concentration)


Thanks to your suggestion, so far my final answer is 7.48447 ppm (unknown concentration)

However, I am just wondering why Prof. gave the graph of excitation spectrum of A. One reason I can think of is that I use the highest intensity which is 100 and then plug it in the equation to find x. If I did that, my final concentration would be 70. Is it possible to do so

Furthermore, the second question is "If a synchronous experiment is to be performed, what would be the offset that you would use?
Thank you in advance for your help

Answer 1

I don't know the answer to your first question since I don't know what your prof had in mind. The information and questions after that is out of my league. I've never done any work with synchronous spectra; in fact, I don't know what it is. Sorry about that.