Please help, I can't do this and its stressing me out ?!?! A 25.0 mL aliquot of an aqeuous quinine solution was diluted to 50.0mL and was found to have an absorbance of 0.832 at 348nm when measured in 2.00cm cell. A second 25.0mL aliquot was mixed with 10.0ml of a solution containing 23.4ppm of quinine, and after dilution to 50.0ml, had an absorbance of 1.220 (2.00 cm cell path). Calculate the concentration in parts per million of quinine in the sample.

1 answer

The standard is in the second part.
A = abc
A = 1.220
a = ?
b = 2
c = 23.4 ppm
a = A/bc = ?

Then for the unknown,
A = abc.
You know A = 0.832, a(from the calculation above), and b = 2 cm. Solve for c of the unknown in ppm.
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