Asked by Suzy
                Can you tell if Beer's Law holds for your stock solutions as well as in the calibration range? Why or why not?
(P.S. this is a lab question and Beer's Law equation is: A(absorbance) = a(absorptivity) x b(path length) x c(concentration))
            
        (P.S. this is a lab question and Beer's Law equation is: A(absorbance) = a(absorptivity) x b(path length) x c(concentration))
Answers
                    Answered by
            DrBob222
            
    I'm confused by the language of "Can you tell if". Did you run any experiments specifically for this? If the dilute solutions follow Beer's Law and the stock solutions are not too concentrated, they may follow Beer's Law also. Generally, however, concentrated solution do not follow Beer's Law and the more concentrated they are the more they deviate from Beer's Law.
    
                    Answered by
            Suzy
            
    Yes we did ran an experiment for this but I was confused as to how I could find the stock solution absorbance. Here's the data I had obtained:
- Unknown solution absorbance: 0.271
- Kool-Aid flavor: Cherry
- Wavelength = 470nm
- Mass of mix 1 transferred = 4.1378g
- Volume of stock solution = 1000mL
    
- Unknown solution absorbance: 0.271
- Kool-Aid flavor: Cherry
- Wavelength = 470nm
- Mass of mix 1 transferred = 4.1378g
- Volume of stock solution = 1000mL
                    Answered by
            Suzy
            
    And the net weight for that is 3.6g (I'm not sure how to use it)
    
                    Answered by
            DrBob222
            
    I would have measured the absorbance.
Did you have a working curve; e.g., absorbance measurements of several solutions of different concentrations.
They look like this.
https://www.google.com/search?q=graph+beer%27s+law&client=firefox-a&hs=bnu&rls=org.mozilla:en-US:official&channel=sb&tbm=isch&imgil=mdlUrxcchZpe8M%253A%253BW7kfg84I6VBiYM%253Bhttp%25253A%25252F%25252Fwww.ic.sunysb.edu%25252FClass%25252Fche133%25252Flectures%25252Fbeerslaw.html&source=iu&pf=m&fir=mdlUrxcchZpe8M%253A%252CW7kfg84I6VBiYM%252C_&usg=__qd4xkMciBFeGHOfXSwOclBn7coE%3D&biw=1024&bih=609&ved=0CCsQyjc&ei=0k1uVJT8DIGNgwTk74KoCg#facrc=_&imgdii=_&imgrc=mdlUrxcchZpe8M%253A%3BW7kfg84I6VBiYM%3Bhttp%253A%252F%252Fwww.ic.sunysb.edu%252FClass%252Fche133%252Flectures%252Fbeerdimer.jpg%3Bhttp%253A%252F%252Fwww.ic.sunysb.edu%252FClass%252Fche133%252Flectures%252Fbeerslaw.html%3B889%3B607
    
Did you have a working curve; e.g., absorbance measurements of several solutions of different concentrations.
They look like this.
https://www.google.com/search?q=graph+beer%27s+law&client=firefox-a&hs=bnu&rls=org.mozilla:en-US:official&channel=sb&tbm=isch&imgil=mdlUrxcchZpe8M%253A%253BW7kfg84I6VBiYM%253Bhttp%25253A%25252F%25252Fwww.ic.sunysb.edu%25252FClass%25252Fche133%25252Flectures%25252Fbeerslaw.html&source=iu&pf=m&fir=mdlUrxcchZpe8M%253A%252CW7kfg84I6VBiYM%252C_&usg=__qd4xkMciBFeGHOfXSwOclBn7coE%3D&biw=1024&bih=609&ved=0CCsQyjc&ei=0k1uVJT8DIGNgwTk74KoCg#facrc=_&imgdii=_&imgrc=mdlUrxcchZpe8M%253A%3BW7kfg84I6VBiYM%3Bhttp%253A%252F%252Fwww.ic.sunysb.edu%252FClass%252Fche133%252Flectures%252Fbeerdimer.jpg%3Bhttp%253A%252F%252Fwww.ic.sunysb.edu%252FClass%252Fche133%252Flectures%252Fbeerslaw.html%3B889%3B607
                    Answered by
            Suzy
            
    I wanted to get that graph but I didn't know how to find the concentration. Only way I can plot it is using concentration and absorbance
    
                    Answered by
            DrBob222
            
    Isn't that all you need? Plot A vs C. You get concentrations from the volume of the stock solution diluted to x mL.
    
                    Answered by
            Suzy
            
    Well i have the dilution (mL/100mL) and absorbance. So to get concentration do I take the dilution and divide it by the volume of stock solution I used? 
    
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