1. She has a brother.

2. He has a brother.
3. It has a brother.
[Can we use all the expressions? Does "It' refer to "She" or "He"? When 'It' refers to an animal, can we use Sentence 3?]

2 answers

1 and 2 are fine.

3 would be fine if it's clear that "it" refers to a cat or dog or other animal, I guess.
1 and 2 are good. 3 is wrong.
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