1. Preparation of Plant Extracts:

- Fresh leaves of Tithonia diversifolia were cut and thoroughly washed with distilled water.
- Leaves were boiled, and the extraction process was conducted using DCM and ethanol.
- Extracts were prepared and stored for further use.
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Fresh leaves of Tithonia diversifolia were carefully cut and thoroughly washed with distilled water. Subsequently, the leaves were boiled, and the extraction process was carried out using dichloromethane (DCM) and ethanol. The resultant extracts were then prepared and stored for future use.