d) in situ hybridization
1) a), b), and d)
2) transcriptional fusion with GFP gene and translational fusion with GFP gene
3) b) enhancer trapping
1) A transcriptional fusion of regulatory sequences of a particular gene with a reporter gene results in relatively uniform expression of the reporter gene in all cells of an organism, whereas a translational fusion with the same gene shows reporter gene expression only in the nucleus of a specific cell type.
Choose the correct possible biological causes for the difference in expression patterns of the two transgenes. Select the three correct answers.
a) Expression of the gene is controlled transcriptionally.
b) Expression of the gene is controlled translationally.
c) Expression of the gene is controlled post-translationally.
d) Expression of the gene is controlled post-transcriptionally.
e) Expression of the gene is controlled by modification of DNA.
2) The CBF genes of Arabidopsis are induced by exposure of the plants to low temperature.
How would you examine the temporal and spatial patterns of expression after induction by low temperature?
Select the two correct answers.
transcriptional fusion with GFP gene
translational fusion with GFP gene
translational fusion with lacZ gene
transcriptional fusion with lacZ gene
3) Can you design a method that would indicate these changes in gene expression in a way that a farmer could recognize them by observing plants growing in the field?
a) fusion with GFP gene
b) enhancer trapping
c) fusion with lacZ gene
1 answer
1 answer