The absence of the wolf would have the greatest negative effect on the ecosystem.
Wolves are apex predators that help control the populations of both foxes and deer. Without wolves, the populations of these prey species could increase unchecked, leading to overgrazing and negative impacts on vegetation and other species within the ecosystem. Additionally, since foxes rely on rabbits for food, the absence of wolves could also indirectly affect rabbit populations. Therefore, the removal of wolves would have a cascading negative effect on the entire ecosystem.